Cloning and expression of a novel R-2-haloacid dehalogenase from Pseudomonas sp. 2-CPA-26 based on the phylogeny-based enzymatic substrate specificity prediction

摘要：

Halogenated organic compounds have been widely used as pesticides, herbicides and solvents. Dehalogenases are microbial enzymes that catalyze the critical step in the breakdown of priority halogenated organic pollutants (Janssen, 2007). Among these, 2-haloacid (aHA) dehalogenases (EC 3.8.1.2) have been extensively studied and classified into two phylogenetic groups, I and II. Group II aHA dehalogenases (Kurihara et al. 2008), which are better characterized both biochemically and structurally, specifically catalyze the dehalogenation of S-2-haloalkanoic acids to the corresponding R-2-hydroxyalkanoic acids. However, some group I aHA dehalogenases are able to process both R-and S-haloacids, whilst others can only process the R-enatiomer.

关键词： R-2-haloacid dehalogenase Pseudomonas sp. 2-CPA-26 Substrate specificity Phylogeny

作者: Lin Chunjiao Wu Jianping Xu Gang Zhao Wenting Yang Lirong

作者单位: Department of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China

会议类型: 国际会议

会议名称: The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)

会议地点: 成都

会议语种:英文

页码: 56-57

在线出版日期: 2010-11-05（万方平台首次上网日期，不代表论文的发表时间）