Characterization of Debranching Enzymes for Glycogen Metabolism in Vibrio vulnificus
Vibrio vulnificus is a rod-shaped, gram-negative halophilic marine bacterium, which can cause septicemia and wound infection in human when contaminated raw seafood is taken. The bacterium experiences a life cycle between nutrient rich hosts and poor aquatic environment. Glycogen is the major energy storage molecule and carbon source for almost all types of organisms including bacteria. The molecule has also significant impact on pathogens since those capable of accumulating more glycogen would have higher chance to survive for longer time in poor environment, compared to others that cannot. Glycogen is polysaccharide with glucose residues linked linearly by a-1,4-glycosidic bonds and branched by a-l,6-glycosidic bonds. In general, bacterial glycogen metabolism pathway include the glgC (glucose-1-phosphate adenylyltransferase), glgA (glycogen synthase), glgB (glycogen branching enzyme) genes encoding enzymes for glycogen synthesis and the glgP (glycogen phosphorylase) and glgX(glycogen debranching enzyme) genes encoding enzymes for glycogen degradation. In agar plate assay of glycogen staining colonies by iodine vapor, the cells of V.
Min-Ju Song Yu-Rhee Choi Hae-Jin Kim Jung-Wan Kim
Division of Life Sciences,University of Incheon,Incheon,Korea
国际会议
The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)
成都
英文
108-109
2010-11-05（万方平台首次上网日期，不代表论文的发表时间）