Enhanced Production of Recombinant Thermobifida fusca Cutinase by TIR Degeneracy Mutagenesis

摘要：

Introduction As the threshold of an intracellular translation process, the Translational Initial Region (TIR) plays a constant key role in determining the whole efficiency of protein translation. Tiny alternations in TIR which extends from immediately from upstream of the Shine-Dalgarno sequence to approximately twenty nucleotides downstream of the initiation codon were reported to change the subsequent downstream translation process (1). Such property provides not only the importance of TIR to the global translation efficiency, but also a powerful potential tool for optimization of a specific recombinant protein. In present study, degeneracy mutagenesis was applied in pelB which is the most widely used signal peptide in E.coli for extracellular production of recombinant protein to enhance the expression level of cutinase from Thermobifida fusca.

作者: Zhiguo Liu Sheng Chen Dan Wu Jian Chen Jing Wu

作者单位: State Key Laboratory of Food Science and Technology,iangnan University,1800 LihuAve.,Wuxi,Jiangsu 214122,China School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education,Jiangnan University,1800 LihuAve.,Wuxi,Jiangsu 214122,China

会议类型: 国际会议

会议名称: The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)

会议地点: 成都

会议语种:英文

页码: 131-132

在线出版日期: 2010-11-05（万方平台首次上网日期，不代表论文的发表时间）