Strategies for achieving high-level expression and purification of thermostable archaeal genes in Escherichia coli
Introduction Hyperthermophiles are important resources of thermostable proteins, having the advantage of tolerance under harsh conditions. Escherichia coli expression system is an advanced system and many techniques have been made available to enhance the efficiency of expression of heterologous genes. Several genes from hyperthermophilic archaeon Sulfolobus have been successfully expressed in E. coli into soluble proteins and their activities and functions were characterized. However, large quantities of the genes from Sulfolobus are still difficult to be expressed in E. coli unlike the genes from mesophiles due to the formation of insoluble particles collectively termed inclusion bodies or translational stalling by the difference of codon usage of mRNA between hyperthermophilic archaea and E. coli host. Especially, S. acidocaldarius has a high AT-rich genome of about 64％, which has made heterologous expression of AT-rich genes in E. coli extremely difficult and inefficient.
Sungmin Hwang Jaeho Cha
Department of Microbiology,College of Natural Sciences,Pusan National University,Busan 609-735,Republic of Korea
国际会议
The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)
成都
英文
143-144
2010-11-05（万方平台首次上网日期，不代表论文的发表时间）