Studies on nrDNA ITS in species-level identification and evolutionary in Pasqueflower
The genus Pulsatilla, commonly known as Pasqueflower, is a member of the family Ranunculaceae and is a representative prescription of herbal medicine for the treatment of diseases caused by bacteria, having prominent curative effects on enteritis and bacillary dysentery.(Cummings et al., 1997) Pasqueflower grows naturally five species(Pulsatilla cernua, Pulsatilla davurica, Pulsatilla koreana Nakai, Pulsatilla nivalis Nakai and Pulsatilla tongkanggenesis Y,N.Lee et T.C.Lee) in Korea.(Lee, 1993) Pasqueflower usually identified from their morphological features in limited areas of Korea, however, it is more difficult to discriminate by their morphological or chemical features because these features overlap (Takeda et al., 1995). Concerning genetic identification, it has been reported that the three species of medicinal Pulsatilla can be distinguished by the restriction fragment length polymorphism (RFLP) analysis of chloroplast DNA. However, identification by RFLP analysis is not effective for crude drugs because the long length of DNA fragments that are effective for RFLP analysis are not obtained from crude drugs. DNA fragments of the crude drugs are usually fractionated and become too short.
Jae-Hak Kim Xiao-Na Yu Soon-Kwan Hong
Department of BioHealth Technology,Kangwon National University,Chuncheon,Kangwon-Do,200-701,Korea Department of BioHealth Technology,Kangwon National University,Chuncheon,Kangwon-Do,200-701,Korea In
国际会议
The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)
成都
英文
153-154
2010-11-05（万方平台首次上网日期，不代表论文的发表时间）