Label-free biomarker detection from whole blood
Label-free nanosensors can detect disease markers to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. However, detecting these biomarkers in physiological fluid samples is difficult because of ionic screening. Here, we overcome this limitation by using distinct components within the sensor to perform purification and detection.1 A microfluidic purification chip captures multiple biomarkers simultaneously from blood samples and releases them, after washing, into purified buffer for sensing by a silicon nanoribbon detector. This two-stage approach isolates the detector from the complex environment of whole blood, and reduces its minimum required sensitivity by effectively preconcentrating the biomarkers. We show specific and quantitative detection of two model cancer antigens from a 10 uL sample of whole blood in less than 20 minutes.
Eric Stern Aleksandar Vacic Nitin K. Rajan Jason M. Criscione Jason Park Tarek M. Fahmy Mark A. Reed
Yale University, School of Engineering and Applied Science, Departments of Biomedical Electrical and New Haven, CT 06511 Yale University, School of Engineering and Applied Science, Departments of Biomedical Cheraical Eng Applied Physics, New Haven, CT 06511
国际会议
上海
英文
1392-1393
2010-11-01（万方平台首次上网日期，不代表论文的发表时间）