A tool for assembling the metagenomic shotgun sequencing reads
Ever since the initial shotgun sequencing projects of metagenomes were launched, the assembly process has been applied on many of them, owing to the more valuable genomic content it can provide. Assembly of reads can improve the quality of the materials used in down-stream analysis such as gene start prediction and gene finding. Recently, some metagenome projects that obtained their data through pyrosequencing, mainly the 454 sequencing, also assembled shorter reads (100-400 bp) instead of Sangers longer reads (600-1000 bp), while some of them only compared the reads against the reference database as the basis of further genomic, taxonomic or functional analysis, mainly due to the poor quality of the assembly of the short reads. However, as the pyrosequencing tech gradually improve its ability of generating longer reads, assembly process will become normal in the metagenomic research.
Binbin Lai Ruogu Ding Yang Li Huaiqiu Zhu
Department of Biomedical Engineering, College of Engineering, and Center for Theoretical Biology Peking University, Beijing, 100871, China
国际会议
杭州
英文
25-27
2010-10-01（万方平台首次上网日期，不代表论文的发表时间）