Comparison of 12 different melting temperature calculation methods for short oligonucleotides

摘要：

Melting temperature (Tm) of oligonucleotide is the key factor for the success of several popular molecular techniques, such as quantitative polymerase chain reaction (PCR), multiplex-PCR and DNA microarrays of short sequences with fixed length. Although there are more than 20 methods or variants available for prediction of the Tm value, the difference of predicting accuracy is quite large and brings much trouble when designing a suitable PCR primer or probes for DNA microarray. Therefore, its important to compare these methods and find out which is the most accurate predicting method.

作者: Wubin Qu Yiming Lu Yang Zhou Chenggang Zhang

作者单位: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Beijing, 100850, China

会议类型: 国际会议

会议名称: 第四届全国生物信息学与系统生物学学术大会

会议地点: 杭州

会议语种:英文

页码: 58-59

在线出版日期: 2010-10-01（万方平台首次上网日期，不代表论文的发表时间）