Histochemical Localization of Endogenous Peroxidase in the Gill, Small Intestine, and Liver of Carassius Auratus
Peroxidases (EC 1.11.1.7) catalyze the oxidation of phenolic compounds using either hydrogen peroxide (H2O2) or organic peroxides as the oxidizing agent. Peroxidases play a significant role in the antioxidant defense systems. In immunohistochemistry, endogenous peroxidase acitivity is usually blocked with the saturating amounts of H2O2. In the present studies, the distribution of endogenous peroxidase was investigated in the gill, small intestine, and liver of Carassius auratus. The results indicate that peroxidase distributes widely in the branchial filaments and intestinal epithelial cells of Carassius auratus. However, there are fewer granules of peroxidase in the liver cells. The results indicate that it is important to block endogenous peroxidase acitivity in these tissues of Carassius auratus with immunohistochemistry technology.
peroxidase Carassius auratus gill small intestine liver
Dongwu Liu Xuemei Liu Zhiwei Chen Hongbing Tian
Analysis and Testing Center, Shandong University of Technology 255049 Zibo, China Department of Nephrology, the Affiliated Hospital of Qingdao University Medical College 266003 Qingd School of Life Sciences, Shandong University of Technology 255049 Zibo, China
国际会议
武汉
英文
802-804
2010-06-06（万方平台首次上网日期，不代表论文的发表时间）