Tow steps biohydrogen production: biomass pretreatment and fermentation
This paper investigated the pretreatment of cornstalk and integrated dark-photo fermentation for hydrogen production. Five parameters of the pretreatment experiments, including NaOH concentration, temperature, residence time, and dosage of cellulase and xylanase, were optimized through the L25 (5∧5) orthogonal test. The optimal NaOH concentration, temperature, residence time, and dosage of cellulase and xylanase were 0.5wt％, 115°C, 3 h, 0.08g/g cornstalk, 0.08g/g cornstalk, respectively. Under the optimal conditions, 0.31g glucose/g cornstalk was obtained. The two-step fermentation consisted of dark fermentation and photo fermentation. The pretreated cornstalk was used as the substrate for dark fermentation, with cow dung as the inoculum. Then the effluents of dark fermentation were employed as the substrate for photo fermentation by photosynthetic bacteria. H2 yield of dark fermentation was 116.7 mL/g cornstalk, with H2 concentration of 41％. After photo fermentation, the total H2 yield increased to 294 mL/g cornstalk.
pretreatment dark fermentation photo fermentation biohydrogen
C.Ma H.H.Yang L.J.Guo
State Key Laboratory of Multiphase Flow in Power Engineering, Xian Jiaotong University, China
国际会议
西安
英文
1060-1065
2009-07-11（万方平台首次上网日期，不代表论文的发表时间）