Evaluation of Transgenic Sweet Orange (Citrus sinenis Osbeck)Containing Bivalent Anti-bacterial Peptide Genes ShivaA and CecropinB
The axillary buds from mature branch of Jincheng Orange (Citrus sinenis Osbeck) and Newhall navel orange (C. sinenis Osbeck) were used for Agrobacterium-based transformation. PCR and Southern blotting analysis verified that the ShivaA and CecropinB genes had been integrated into the genome of transgenic plants. The transgenic plants began to blossom and to bear fruits in the 3rd year after grafted on trifoliate orange (Poncirus trifoliata Raf) rootstocks in greenhouse. Through evaluating in greenhouse, no significant differences were observed on the total soluble solids, total acidity, reducing sugar and fruit shape index of characteristics between transgenic and non-transgenic mature lines. Extract of transgenic citrus leaves could suppress the growth of citrus canker pathogen (Xanthomonas axonopodis pv. citri) in vitro, which suggests that the ShivaA and CecropinB had expressed efficiently in some transgenic citrus and exhibited anti-bacterial activity. By artificially inoculating with the canker pathogen in greenhouse and field, the different resistance capabilities to canker were observed among transgenic and control lines, and we had selected 11 significantly enhanced canker resistance lines from 40 transgenic lines.
Mature axillary bud Transformation Horticultural characteristics Canker resistance
Yongrui He Aihong Peng Lanzhen Xu Tiangang Lei Xiaofeng Liu Lixiao Yao Shanchun Chen Shirong Jia Yixiong Tang
Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, P. R. China N Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. C
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
272-280
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）