Systemic Acquired Resistance (SAR) in Citrus: Genetic Transformation with SAR Genes for Resistance to Huanglongbing and Canker
Systemic acquired resistance (SAR) is the activation of a plants defense mechanism leading to the induction of a systemic resistance to subsequent pathogen infection. It is widely known that salicylic acid (SA) is a key component in the signal transduction pathways. It is expected that induction of SAR in citrus following an initial infection by pathogens, including the gram negative bacteria responsible for HLB or canker, will result in the development of resistance to subsequent disease infections. We used several SAR genes, including the Arabidopsis NPR1 gene and the tobacco Salicylic Acid Binding Protein2 (SABP2) gene and have constitutively expressed them in sweet orange plants via Agrobacterium mediated transformation. Genetically modified plants containing the genes driven by a phloem specific Arabidopsis sucrose synthase promoter have also been produced. Several of these plants have been multiplied for disease resistance evaluation.
Agrobacterium Arabidopsis Tobacco Canker Citrus transformation HLB Green Fluorescent Protein (GFP) Candidatus Liberibacter asiaticus Xanthomonas citri ssp. Citri
M. Dutt G. Barthe and J. W. Grosser
Citrus Research and Education Center, University of Florida/IF AS, 700 Experiment Station Road, Lake Alfred, FL 33850 U. S. A
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
289-294
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）