cDNA-AFLP Identifies Differently Expressed Transcripts of a Novel Late-ripening Citrus sinensis Mutant Potentially elated to Fruit Ripening
Fengjiewancheng (FJWC) is a late-ripening bud sport of Fengjie72-1 navel orange (Citrus sinensis Osbeck). In the present research, using cDNA-amplified fragment length polymorphism, we compared the transcript difference between fruits of the two varieties during ripening. As a result, about 60 000 clear transcript-derived fragments (TDFs) were generated with band sizes ranging from 50 to 500bp, of which 127 TDFs were down regulated (88.2％) and 17 were up-regulated (11.8％) in FJWC while compared with FJ72-1. Blast annotation indicated that 39 uniTDFs, which mainly located in mitochondria (26％)and membrane system (44％), were assigned with putative function in signal transduction, control of gene expression and carbohydrate biosynthesis, involving in extensive biological processes such as response to stimulus (12％), transport (12％), cellular metabolic process (12％), post-translational protein modification (12％), micromolecule biosynthesis (12％), transcription (10％), cellular localization (10％), phosphate metabolic process (10％)and developmental process (10％ ). In addition, some TDFs showed relatively high identities with known functional genes and had differential expression patterns in fruit pulp or peel between FJWC and FJ72-1 during fruit ripening, implying a potential role in the formation of the late-ripening phenotype.
Bud sport CDNA-AFLP Citrus sinensis Fruit ripening
Yongzhong Liu Andan Zhu Junli Ye M. N. R. Baig Xiuxin Deng
National Key Laboratory of Crop Genetic Improvement College of Horticulture and Forestry Science,Hua National Key Laboratory of Crop Genetic Improvement College of Horticulture and Forestry Science, Hu National Key Laboratory of Crop Genetic Improvement College of Horticulture and Forestry Science, Hu National Key aboratory of Crop Genetic Improvement College of Horticulture and Forestry Science, Hua National Key Laboratory of Crop Genetic Improvement College of Horticulture and Forestry Science, Hu
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
303-311
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）