Biochemical and Molecular Analysis of Fruits on Comune Clementine and Its Late-ripening Mutant Tardivo
Peel color is one of the main features affecting citrus quality. In this work we characterized the fruit development and ripening of two clementine clones, Comune , a widespread cultivar, and one of its natural mutations, Tardivo , which differ by a delayed color-break and extended harvest stage. Morphological, chemical and molecular analyses were carried out on fruits of both genotypes during the whole maturation process. Analysis showed that mutation did not affect ripening characteristics such as juice acidity and TSS. However, biochemical and molecular analyses revealed marked differences in the flavedo regarding carotenogenesis and chlorophyllase gene expressions. Carotenoid showed quantitative differences at biochemical and molecular level. Results demonstrated that the mutation in Tardivo influenced the transcriptional activation of phytoene synthase (PSY) , a key step in carotenoid biosynthesis. The differential PSY expression led to a significant quantitative difference in phytoene accumulation between the two genotypes. Also, Tardivo showed delayed accumulation of carotenes, lutein and β,βxanthophylls. The differential expression of genes involved in ethylene biosynthesis and perception suggested differential responses to ethylene signalling between the two genotypes. The analysis added new information to better understand the complex process of ripening in citrus.
Citrus Chlorophyllase Carotenogenesis HPLC Ethylene
Giuseppina Las Casas Gaetano Distefano Marco Caruso Stefano La Malfa Alessandra Gentile Eugenio Tribulato Paolo Rapisarda
Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari, University of Catania, 95123 Cat C. R. A. - Centro di ricerca per Iagmmicoltura e le colture mediterranee di Acireale, Catania, Ita
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
340-348
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）