Regeneration of Citrus Genotypes by Stigma/Style Somatic Embryogenesis in Algeria; Preliminary Results
Stigma/style somatic embryogenesis proved to be an excellent method for the regeneration of most Citrus spp. , with the exception of Clementines, without inducing somaclonal variations in regenerants. In this study, such method was applied onto a citrus collection at ITAF, Algeria. The main citrus local and international varieties were chosen for regeneration by stigma/style somatic embryogenesis. Explants were cultured in media I and II to induce the formation of somatic embryos. In both media, MS basal salts, vitamins, sucrose and malt extract were used. In medium II, 3 mg/L of the 6-benzylaminopurine growth regulator was also added. All explants of C. Union and C. sinensis produced callus at the cut end of styles, about 8 days after culture initiation. Most of the tested genotypes proved to be able to regenerate somatic embryos in a different period of time (25-90 days) , which were cultured in a single tube before in vivo acclimatization.
Stigma/Style culture Somatic embryogenesis Algeria Results
Meziane M. Boudjeniba M. Frasheri D Onghia A. M. D Carra A Carimi F
University Hassiba Ben Bouali, Hay Salem, route national 19 (02000) Chief, Algerie Laboratoire des Cultures in vitro. Ens Kouba, Alger, Algeria Centre International de Hautes Etudes Agronomiques Mediterraniennes (CIHEAM) /Mediterranean Agronomi Institute of Plant Genetics/CNR, Research Division of Palermo, Corso Calatqfimi 414, 90129 Palermo,
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
357-361
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）