Cloning and Sequence Analysis of Expansin Genes in Citrus Fruits

摘要：

Degenerate oligonucleotide primers were designed on the basis of conserved domain of known expansions to amplify cDNA fragment of 531 bp in Citrus peel by RT-PCR. According to the cDNA fragment sequence, the 5 end and 3 end fragment sequences were obtained by RACE technology. The full length cDNA of expansion gene, named Ct-Exp 1, was cloned. Ct-Exp 1 was composed of 1 196 bp, encoding 246 amino acids. Eight cysteine residues and 4 tryptophan residues, which are supposed to be the characteristics of expansins, are conserved in Ct-Exp 1, and it contains histidine function region. Its BLAST search against GenBank database showed that it showes 88％ , 88％ , 87％ and 86％ , 85％ homology with the sequences of EXP proteins in Malus X domestica, Dimocarpus longan, Prunus cerasus, Vitis labruscax Vitis vinifera, Prunus persica.

关键词： Citrus Expansin cDNA cloning Sequence analysis

作者: Li Juan Chen Jiezhong Zhang Panpan Yao Qing Lu Wangjin

作者单位: College of Hotirculture, South China Agricultural University, Guangzhou 510642. P. R. China Co College of Hotirculture, South China Agricultural University, Guangzhou 510642. P. R. China

会议类型: 国际会议

会议名称: 11th International Citrus Congress(第11届国际柑橘大会)

会议地点: 武汉

会议语种:英文

页码: 378-382

在线出版日期: 2008-10-01（万方平台首次上网日期，不代表论文的发表时间）