Application of SSR Molecular Marker in the Identification of Citrus Varieties
Simple sequence repeat (SSR) marker was used to study the relationships of the sixty three of citrus collections. A total of fifteen SSR pairs of primer were screened out of 22 primer pairs. A total of 277 amplified bands were detected, among which 267 polymorphic bands with averages of 6～36 bands produced by each pair. The results showed that all the cultivars among the collections shared more than 0.7 of genetic similarity, of which these were 0.964, 0.988 and 0. 913 of genetic similarity coefficient for the different varieties ofsweet orange (Citrus sinensis) , among different varieties of satsuma (C. unshiu) and among different varieties ofponkan (C. reticulata) , respectively. Nevertheless, it was existed wide variation range of genetic similarity among other citrus hybrids. The obvious genetic divergence was observed among species or cultivars of Fortunella crassifolia Swingle, C. limon Burm. , Citrus aurantifolia Swingle, C. grandis Osb. , C. reticulata Blanco and C. sinensis Osb. , respectively. It could be differentiated from each other except Seike navel and Shirayanagi navel, Gailiangcheng and Tarocco orange Xinxi , Ponkan 56-17 and Yanxiwanlu ponkan.
Genetic similarity Phylogenetic relationship
He J Chen KL Liu JJ Li HW Wang JH Guan B
Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
388-391
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）