Adventitious Shoot Regeneration from Five Commercial Citrus Cultivars Using a Range of Explant Types

摘要：

The parameters for optimum regeneration of five commercial citrus cultivars were tested using a range of explants. Different optimum medium for shoot regeneration of the citrus cultivars Sihuitianju (Citrus retkulata Blanco.), Shatangju (C. reticulata Blanco.), Ponkan (C. reticulata Blanco.), Gonggan (natural hybrid cultivars of C. sinensis X C. reticulata Blanco.)and Anliucheng (C. sinensis Osbeck) were achieved using epicotyl segments, hypocotyl segments and cotyledon as explants respectively. The optimum medium for shoot regeneration of Sihuitianju was MT medium supplemented with 5 mg/L 6-BA and 0.5 mg/L IBA; the optimum medium for Shatangju was MT medium supplemented with 2. 5 mg/L 6-BA and 0. 5 mg/L NAA; the optimum medium for f Ponkan was MT medium supplemented with 0.5 mg/L NAA; the optimum medium for Gonggan was MT medium supplemented with 7. 5 mg/L 6-BA and the optimum medium for Anliucheng was MT medium supplemented with 2.5 mg/L 6-BA and 0.5 mg/L IBA. Regeneration from epicotyl proved to a promising explant source while cotyledon proved to be difficult from all explant materials. This system thus provides a convenient method that could be adopted for commercial citrus in vitro propagation or genetic transformation of commercial citrus varieties.

关键词： Citrus Shoot regeneration Epicotyl Hypocotyl Cotyledon

作者: Tang Y Qin YH Hu GB Lin SQ

作者单位: College of Horticulture, South China Agricultural University, Guangzhou, Guangdong, 510642,P. R. C College of Horticulture, South China Agricultural University, Guangzhou, Guangdong, 510642,P. R. C College of Horticulture, South China Agricultural University, Guangzhou, Guangdong, 510642,P. R. C

会议类型: 国际会议

会议名称: 11th International Citrus Congress(第11届国际柑橘大会)

会议地点: 武汉

会议语种:英文

页码: 398-403

在线出版日期: 2008-10-01（万方平台首次上网日期，不代表论文的发表时间）