Molecular Cloning and Bioinformatic Analysis of CBF-like Genomic DNA and Upstream Regulatory Elements in Rough Lemon (Citrus jambhiri Lush.)
In this study, genomic DNA fragment and upstream regulatory sequence of CBF-like gene, the key gene related to cold responses, were isolated from rough lemon (Citrus jambhiri Lush.), then analyzed by a series of bioinformatic tools for further functional characterization. Results showed that there were no introns in CBF-like gene from rough lemon, the same as the other species previously reported. Furthermore, signature sequence and AP2 DNA binding domain, highly conserved in all CBF family members, were observed in rough lemon CBF-like protein sequence by multiple alignment analysis with those from other species. Many stress-related cisacting elements, such as MYB, MYC, and ABRE, were existed in rough lemon CBF-like gene upstream regulatory sequence by PLACE algorithm predictions. In addition, a potential transcription start site was predicted by NNPP software. It was expected that this study could provide some practical and theoretical evidences for molecular breeding of transgenic coldtolerant citrus, and contribute to understanding molecular mechanism underlying cold acclimation, CBF regulon network and cross-talk under environmental stress conditions in citrus.
Rough lemon CBF-like Regulatory sequence Molecular cloning Bioinformatic analysis
He LG Wang HL Zhao YJ Xu M Liu DC Sun ZH
College of Forestry Science and Horticulture, Huazhong Agricultural University, Wuhan,430070,P. R. China
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
410-417
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）