Effects of X-irradiation on Scoparone Levels in ClemenulesClementine Mandarin Flavedo
Phytoalexins are frequently produced in infected tissues of plants. It has been demonstrated that phytoalexins such as scoparone (6, 7 dimethoxycoumarin) play an important role in the resistance of citrus fruit against fungal pathogens. Effects of X-ray irradiation (at doses of 510 and 875 Gy) on scoparone level in Clemenules Clementine rind were evaluated in this study. After irradiation, half of mandarins were stored at 20℃ for 7 or 14 days and the other half were stored at 5℃ and 90％ RH for 1 or 2 months. For each storage condition, there was a control mandarin group that was not irradiated, a group treated at 510 Gy and another group treated at 875 Gy. Following these treatments, the level of scoparone accumulated in the flavedo tissue was determined by HPLC analysis using a fluorescence detector. X-irradiation at 875 Gy followed by storage at 20℃ during 14 days was the most effective treatment in stimulating the phytoalexin biosynthesis in the flavedo. The level of phytoalexin induced by this combination reached 24ppm (24μ,g/g dry mandarin flavedo) , twenty- five times higher than that in the flavedo of nonirradiated Clementines kept in the same storage conditions. Fruit irradiated at the dose of 510 Gy and stored at 201 during 7 days presented 19 ppm of scoparone. The levels of scoparone in Clementines stored for 1 or 2 months at 5℃. were very low. Irradiation at applied doses did not modify the levels of vitamin C or flavanone glycosides on the citrus juice.
Clemenules Flavedo X-irradiation Scoparone
Cristina Rojas-Argudo Lluis Palou Miguel Angel del Rio M. Carmen Gonzalez-Mas Almudena Bermejo Antonio Cano
Centro de Tecnologta Poscosecha Centro de Citricultura y Produccidn Vegetal. Institute Valenciano de Investigacione
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
658-661
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）