Analysis of a Ribosomal RNA Operon (rrn) from Candiatus Liberibacter asiaticus
A 5 005 bp rrn DNA sequence of Candidatus Liberibacter asiaticus was obtained by PCR using primers conserved to Rhizobiaceae of alphaproteobacteria. The rrn locus consisted of a 16S rRNA gene (rrs) , an intergenic transcribed spacer (ITS) containing two genes of tRNAlle and tRNA Ala, a 23S rRNA gene (rrl) , an ITS without tRNA gene and a 5S rRNA gene (rrf). like rrs, rrl and rrf were also related (89％ -90％)to those of the members of Rhizobiaceae. Interestingly, the genes of tRNAlle and tRNAAle were more related to those of nonRhizobiaceae alpha-proteobacteria. The non-tRNA gene regions of the 16S-23S ITS and 23S-5S rRNA ITS did not share significant similarity to any known nonLiberibacter DNA sequences, and could be used for specific and efficient detection of Ca. L. asiaticus.
Citrus Huanglongbing Yellow shoot disease Candidatus Liberibacter asiaticus rrn operon Primer walking
Xiaoling Deng Jianchi Chen Heng Guo Huaping Li
Laboratory of Citrus Huanglongbing Research, Department of Plant Pathology, South China Agricultural San Joaquin Valley Agricultural Sciences Center, USDA-ARS, Parlier, CA, USA
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
1087-1090
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）