In vitro Comparison of Disinfectants and Fungicides Efficiencies for Control of Citrus Green Mold
Citrus green mold, caused by Penicillum digitatum is one of the most economically important postharvest diseases of citrus in Uruguay. Fungicides like imazalil, sodium ortho-phenyl phenate (SOPP) and thiabendazole (TBZ) this latter applied only to fruit for internal consumption are used for disease control. Continuous application of fungicides has led to the appearance of TBZ and imazalil resistant strains and, in a few cases, SOPP resistant ones. In addition, it is well know that the use of fungicides may be risky both for consumer health and for the environment. Therefore, it will be necessary to increase the efficiency of other measures of control as for example the disinfection. This work is aimed to compare the effect of various disinfectants and one fungicide over Penicillum digitatum spores viability in vitro. The products assayed were chlorine-containing compounds, 100 ppm stabilized chlorine dioxide (Tecsa-Clor?), 5 OOOppm chloramine-T (Bac-T?); 200 ppm sodium hypochlorite , and ammonia-containing products, 2 ppm didecyl dimethyll ammonium chloride/alkyl amido propyl dimethyl benzil ammonium chloride, (Tefor?); 120 ppm didecyl dimethyll ammonium chloride (Sporekill? ). The efficiency of fungicide 20 000 ppm SOPP, (Espumer-0 Mercosur?)was also evaluated. For all treatments, a suspension containing 1 X 106 spores/ml of a imazalil resistant strain was prepared and exposed directly to each product solution for either 1, 2 or 4 minutes. Spores were then collected by filtration on a 0. 45μm membrane, resuspended in water, plated on PDA and incubated at 25℃ until colony counting. A significant effect was detected between products, times and in the interaction product and time. The highest efficiency was observed for quaternary ammonium compounds that killed nearly 100％ of the spores for all three exposition times. Exposure to sodium hypochlorite for 2 and 4 minutes eliminated 99.8％ and 99.9％ of the spores, respectively. Tecsaclor? (adjusted to pH 7) and SOPP solutions, reduced 80％ and 90％ of the viable spores after 4 minutes exposure, respectively. All other treatments assayed, including 1 minute exposure to sodium hypochlorite, showed practically no differences from control.
Penicillum digitatum Citrus green mold Chemical control Disinfectants fungicides
Luis Diaz Raquel del Campo Mercedes Peyrou Hector Mara
Direccidn General de Servicios Agrfcolas (M. G. A. P. ) , Milldn 4703, Montevideo, Uruguay Institute de Investigaciones Bioldgicas Clemente Estable (M. E. C) Av. Italia 3318, Montevideo, Ur
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
1157-1162
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）