Microarray Analysis of Gene Expression Profiles in Citrus Fruit Treated with Chitosan
The ability of chitosan, a tasteless polysaccharide obtained by deacetylation of chitin, in inducing resistance against green mould in harvested oranges was evaluated. In chitosan treated oranges the incidence and severity of decay were significantly reduced as compared with control fruits. In order to understand the possible mode of action of chitosan, changes in gene expression profile were detected in albedo and flavedo treated tissues by using a 20K citrus cDNA microarray containing 22 176 citrus Expressed Sequence Tags (ESTs). A total of 70 genes were preferentially expressed in albedo and flavedo treated with chitosan, while 150 genes were preferentially expressed in tissues treated with water. Gene expression changes involved several functional categories including cell defence, transport, protein metabolism, signal transduction and developmental process. On the whole, data indicate that chitosan may reduce the incidence of Penicillium rots in treated oranges by modifying their gene expression profiles.
Citrus fruit Chitosan Gene expression profiles Microarray analysis
Trullo M. C. Merelo P. Schena L. Tadeo F. R. Gonzalez-Candelas L. Talon M. Ippolito A.
Department of Plant Protection and Applied Microbiology, University of Bari, Italy Institute Valenciano de Investigaciones Agrarias (I. V. I. A. )-Centro de Gendmica, Moncada ( Valenc Department of Management of Agricultural and Forest Systems, Mediterranean University of Reggio Cala Consell Superior dInvestigacions Cienttfiques, Instituto det Agroqu mica y tecnologt a de Alimentos
国际会议
11th International Citrus Congress(第11届国际柑橘大会)
武汉
英文
1337-1341
2008-10-01（万方平台首次上网日期，不代表论文的发表时间）