The Preparation of Thermo-sensitive PNIPAAm with the Metal-chelate Affinity Ligand
Poly (N-isopropyl acrylamide), short for PNIPAAm, shows temperature-sensitive features in aqueous solution due to its macromolecular chains having both hydrophilic amide group (-CONH-) and hydrophobic isopropyl group (-CH (CH3)2). Smart polymer can be designed as chemical controllers, sensors, devices for the intelligent film, memory elements, artificial muscles, drug delivery systems. This paper used an approach of copolymerizationchelated metal ions, which randomly synthesized binary linear polymers using isopropyl acrylamide and acrylic acid monomers. So the polymer not only had the temperature-sensitive character but also obtained functional carboxylic groups (-COOH) to chelate metal ions. Then the PNIPAAm-co-AAm binary linear polymer was bonded with metal ions, which obtained both temperature sensitivity and affinity properties simultaneously. We used water as a polymerization solvent, oxidationreduction system (APS) as initiator, TEMED as an accelerator to prepare PNIPAAm-co-AAm. The optimal synthetic conditions, the concentration of monomers is 4％ (accounting for the total mass of solution), the monomer ratio is each 1.0 g NIPAAm needs to add 200 μl AAm, the concentration of APS initiator is 0.24％ (accounting for the total mass of solution), the concentration of TEMED is 0.24％ (v/v, accounting for the total solution volume). The temperature-sensitive polymer with good physical and chemical properties not only has good thermo-sensitive nature, but also has the pH-sensitive properties, which is suitable as the carrier of protein separation and purification. We chose copper ions as a specific ligand. The results indicated that the optimum condition is that pH 4.5, the initial concentration of copper ions is 2.0 g/L, polymer dosage is 0.20 g, and we found the maximum adsorption capacity of copper ions on PNIPAAm-co-AAm was 63.5 mg/g. PNIPAAm-CO-AAm-Cu also has good storage stability.
PNIPAAm AAm temperature-sensitive metal-chelate
SU Sai-nan NIE Hua-li LING Yuan-qing ZHU Li-min
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P. R. China
国际会议
2010 International Forum on Biomedical Textile Materials(2010国际纺织生物医用论坛)
上海
英文
65-69
2010-05-28（万方平台首次上网日期，不代表论文的发表时间）