Enhanced Enzymatic Hydrolysis of Rice Straw by lonic Liquid Pretreatment for Bacterial Cellulose Production
Rice straw is an abundant resource in the earth. It contains nearly 50％ cellulose and hemicellulose. The cellulose and hemicellulose can be hydrolyzed to sugars and are potential cheap carbon sources for bacterial cellulose production. However, the hydrolytic efficiency of rice straw by using enzymes is low due to complex structure and composition in straw. The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride (-AMIMCI) is a low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice straw and to improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows: the dosage of rice straw in AMIMCI, crush mesh of rice straw, pretreatment temperature and time. After the pretreatment with a 3％ (the ratio of rice straw to ionic liquid, w/w) rice straw dosage in AMIMCI at 110℃ for 1 h, the yield of reducing sugar of regenerated rice straw by cellulase hydrolysis was 53.3％, which was two times higher than that of un-treated rice straw (23.7％). The reason why the pretreatment can accelerate enzymatic hydrolysis was discussed. SEM inspection indicated that the surface on the rice straw pretreated by AMIMCI was porous, which improved the enzymatic accessibility of the regenerated rice straw obviously.
ionic liquid rice straw enzymatic hydrolysis
HAN Shi-fen YANG Xue-xia HONG Feng
Group of Microbio/ogical Engineering and Industrial Biotechnology, College of Chemistry, Chemical En Group of Microbio/ogical Engineering and Industrial Biotechnology, College of Chemistry, Chemical En
国际会议
2010 International Forum on Biomedical Textile Materials(2010国际纺织生物医用论坛)
上海
英文
107-112
2010-05-28（万方平台首次上网日期，不代表论文的发表时间）