Adsorption of Papain onto α-Ketoglutaric Acid Modified Magnetic Chitosan Nanoparticles

摘要：

Chitosan (CS)-coated γ-Fe2O3 nanoparticles (MNPs) attached with a biodegradable and eco-friendly biologic reagent, α-ketoglutaric acid (α-KA), was prepared and used as a magnetic nanoadsorbent to separate the papain from aqueous solution. Dynamic adsorption experiments on the magnetic nanoadsorbent were carried out at various pH values, ionic strength and initial papain concentrations to determine optimum magnetic nanoadsorbent adsorption properties. The maximum adsorption capacity for papain was up to 92.3 mg/g. Isotherm study shows that the Freundlich model was able to describe the papain adsorption process with a good fitting. Desorption experiment indicates that the chitosan coated magnetic nanoparticles grafted with α-ketoglutaric acid has high stability and recovery capacity. It is concluded that α-KA-CS-MNPs could be a potential effective adsorbent for the industrial isolation of the valuable proteins.

关键词： magnetic separation α-ketoglutaric acid papain

作者: SONG Ming-min NIE Hua-li ZHOU Yu-ting BRANFORD-WHIT Christopher ZHU Li-min

作者单位: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, Institute for Health Research and Policy, London Metropolitan University, London, UK College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620,

会议类型: 国际会议

会议名称: 2010 International Forum on Biomedical Textile Materials(2010国际纺织生物医用论坛)

会议地点: 上海

会议语种:英文

页码: 130-134

在线出版日期: 2010-05-28（万方平台首次上网日期，不代表论文的发表时间）