Fabrication and Characterization of Gelatin/hyaluronan Scaffolds for Tissue Engineering
(0) A three-dimensional (3-D) cellular scaffold plays a vital role in tissue engineering, providing an extracellular matrix (ECM) analog which functions as a necessary template for host ingrowth and a physical support to guide the differentiation and proliferation of cells into the targeted functional tissue or organ. In this study, a threedimensional scaffold with a porous structure was prepared at -20, -80 or - 196℃ by a rapid freeze-drying method and cross-linked using 1-ethyl 3- (3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) to improve mechanical stability. To obtain the optimum conditions for fabrication of gelatin-hyaluronan (Gel-HA) composite porous scaffolds, the characteristics of scaffolds composed of different ratios of gelatin to hyaluronan were further evaluated. The morphology and structure of porous scaffolds were observed by scanning electron microscopy (SEM). The results demonstrated that all of the Gel-HA scaffolds were highly porous and exhibited oriented microtubule pore structure with different pore size depending on the freeze temperature and the gelatin-hyaluronan ratio. The pore size increased with increasing of the ratio of hyaluronan in scaffolds. Furthermore, the properties of the scaffolds including apparent density and swelling ratio were also studied.
gelatin hyaluronan scaffolds tissue engineering
ZHANG Fan HE Chuang-long
Institute of Biology Science and Biotechnology, Donghua University, Shanghai, 201620, P. R. China
国际会议
2010 International Forum on Biomedical Textile Materials(2010国际纺织生物医用论坛)
上海
英文
166-170
2010-05-28（万方平台首次上网日期，不代表论文的发表时间）