Preparation of Immobilized Protease on the Magnetic Chitosan Microspheres and Its Application on Wool Treatment
Immobilized protease on the magnetic chitosan microspheres was prepared with glutaraldehyde as cross-linking agent. The effects of immobilization conditions, the stabilities and the recovery properties were determined. And then the immobilized protease was applied on the wool fabric and the finishing results were tested. When pH was 7.5, the temperature was 40℃, the concentrations of EDC and glutaraldehyde were respectively 0. 25％ (W/W) and 2. 0％ (W/W), the concentration of enzyme was 0.4％ (V/V), the relative activity of the immobilized protease reached 66％. Compared with native protease, the pH, thermal and storage stabilities of the immobilized protease were greatly improved. In addition, the immobilized protease had desirable reusable and was used for 30 times with about 81％ ratio of activity recovery. The prepared immobilized protease was used for treatment of wool fabric, the area shrinkage rate was 7.1％, the weight loss percentage was 6.4％ and the strength loss percentage was 8.2％. These results confirm that when treating wool fabrics with immobilized protease, by the proper treatment conditions, the good levels of shrink resistance without considerably damaging the fabric can be achieved.
magnetic chitosan microspheres immobilized protease activity recovery stability wcol treatment
GE Feng-yan ZHONG Ye-gui LIU Jing-jing CAI Zai-sheng
Key Laboratory of Textile Science & Technology, Ministy of Education, Donghua Universtiy, Shanghai, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620
国际会议
2010 International Forum on Biomedical Textile Materials(2010国际纺织生物医用论坛)
上海
英文
188-193
2010-05-28（万方平台首次上网日期，不代表论文的发表时间）