Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant pathogens

摘要：

Fluorescent silica nanoprobe as a biomarker for detection has attracted much attention in the field of nano-biotechnology recently but no further research applications using fluorescent silica nanoparticles (FSNP) combined with antibody molecules reported to detect pathogen detection. In this study, silica nanoparticles were prepared using the water-in-oil (w/o) microemulsion method. The silica nanoparticles were circular in diameter of 50 ± 4.2 nm. The organic dye, tris-2, 2 -bipyridyl dichlororuthenium (II) hexahydrate (Rubpy), could be incorporated efficiently into the core of silica nanoparticles. The fluorescence of Rubpy-doped silica nanoparticles was photostable using a collisional quenching fluorescence test. The Rubpy-doped silica nanoparticles were conjugated with the secondary antibody of goat anti-rabbit immunoglobulin G (IgG) and successfully detected plant pathogen such as Xanthomonas axonopodis pv. vesicatoria that causes bacterial spot disease in Solanaceae plant. These results demonstrated that the fluorescence silica nanoprobe biomarker will have been potential for rapid diagnosis applications on plant diseases.

关键词： Fluorescence silica nanoprobe biomarker Fluorescence-linked immunosorbent assay

作者: K.S. Yao S.J Li K.C Tzeng T.C. Cheng C.Y Chang C.Y. Chiu C.Y. Liao J.J. Hsu Z.P. Lin

作者单位: Department of Biotechnology, MingDao University, Changhua, Taiwan Institute of Materials Science and System Engineering, MingDao University, Changhua, Taiwan Department of Plant Pathology, National Chung-Hsing University, Taichung, Taiwan Department of Holistic and Wellness, MingDao University, Changhua, Taiwan

会议类型: 国际会议

会议名称: 第二届多功能材料与结构国际会议

会议地点: 青岛

会议语种:英文

页码: 513-516

在线出版日期: 2009-10-09（万方平台首次上网日期，不代表论文的发表时间）