Preparation, Cytotoxicity and Degradability of Chitosan-Based Thermosensitive Hydrogels as Drug Delivery System
A new thermosensitive hydrogel had been prepared that could be transformed into gel at 37 ℃ from chitosan and a mixture of α- and β-glycerophosphate (αβ-GP). The appearance of hydrogel was compact and corrugated. There was little granule in the appearance of gel loaded with adriamycin and the granules might be crystals of the added model drug. In vitro cytotoxicity of the hydrogel was tested by the MTT method using mouse embryonic fibroblasts (MEF). MEF cultured with leachates of CS-αβ-GP were investigated and the relative growth rate (RGR) was calculated and the cytotoxicity was graded by generally accepted standard. The study of in vitro degradation of CS-αβ-GP hydrogels included hydrolysis and degradation by lysozyme. The CS-αβ-GP thermosensitive hydrogel was degradable in vitro and the degradation rate was faster in lysozyme solution than that in the medium of PBS. So the CS-αβ-GP system had good cell biocompatibility and biodegradability which provided possibilities and foundations for the further research.
Chitosan αβ-GP thermosensitive hydrogel cytotoxicity degradation
Hui Yun Zhou Jian Wang Wei Fen Zhang Ye Wang Xi Guang Chen
The Chemical Engineering & Pharmaceutics College, Henan University of Science and Technology, Luoyan Luoyang Qinling pharmaceutical factory, Luoyang, China, 471031 College of Marine Life Science, Ocean University of China, Qingdao, China, 266003 Department of Medi College of Marine Life Science, Ocean University of China, Qingdao, China, 266003 Institute of Genom College of Marine Life Science, Ocean University of China, Qingdao, China, 266003
国际会议
青岛
英文
1531-1534
2009-10-09（万方平台首次上网日期，不代表论文的发表时间）