Molecular Biology and Proteomics during Somatic Embryogenesis in Dimocarpus longan Lour
Somatic embryogenesis system in longan can serve as a model experimental system of woody plants.Since 2000,on the basis of histological and cytological observations,determination of endogenous hormones and polyamines (Pas),the changes of isozymes and proteins by PAGE,IEF and SDS-PAGE analysis during longan somatic embryogenesis proteomies and molecular biology during longan somatic embryogenesis have been carried out by mRNA differential display, homologous cloning, fluorescence quantitative PCR, 2-DE and bio-mass spectrometry (MALDI-TOF or Q-TOF2) technique,to uncover the molecular mechanism of longan somatic embryo-genesis,and provide scientific evidence for regulating longan embryo development.
real time quantitative PCR gene cloning 2-DE bio-mass spectrometry
Z.X.Lai Y.He Y.T.Chen Y.Q.Cai C.C.Lai Y.L.Lin X.L.Lin Z.Z.Fang
Institute of Horticultural Biotechnology Fujian Agriculture and Forestry University Fuzhou 350002,Fujian China
国际会议
福州
英文
95-101
2008-08-25（万方平台首次上网日期，不代表论文的发表时间）