Long-Term Subculture and Maintenance of Transgenic Resistant Embryogenic Callus in Litchi(Litchi chinensis Sonn.)

摘要：

The effects of subculture method,hygromycin,AgNO3,inoculation quantity and subculture cycle on the growth of litchi trausgenic resistant embryogenic callus (TREC) were studied in this experiment.Meanwhile,the influences of the medium and long-term subculture on the GUS expression of litchi resistant embryogenic callus were carried out.The obtained results showed that litchi TREC could be steadily maintained for a long time when they were alternately subcultured in the MS medium containing 1.0 mg/L 2,4-D,50 mg/L hygromycin and the MS medium supplemented with 1.0 mg/L 2,4-D,100 mg/L inositol and 50 mg/L hygromycin.

关键词： litchi (Litchi chinensis Sonn.) transgenosis resistant embryogenic callus subculture and maintenance GUS assay

作者: C.C.Lai Z.X.Lai Q.L.Sang Z.Y.Huang Q.Huang

作者单位: Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,Ch Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences,Fuzhou

会议类型: 国际会议

会议名称: The Third International Conference Symposium on Longan,Lychee and Other Fruit Trees in Sapindaceae Family(第三届国际龙眼，荔枝及其他无患子科果树学术研讨会)

会议地点: 福州

会议语种:英文

页码: 135-140

在线出版日期: 2008-08-25（万方平台首次上网日期，不代表论文的发表时间）