Changes of APX Isozymes under High-Temperature Stress and Cloning of an Apx Functional Fragment in Embryogenic Callus of Litchi
In this study,the embryogenic callus (EC) of litchi (Litchi chinensis Sonn.) was used as the material.Activity and isozymes bands of APX were analyzed under high-temperature stress.In the meanwhile,a cytosolic apx gene fragment was cloned.Results showed that the expression of apx genes was differently induced by high-temperature stress.The level of APX activity was higher at the 30 and 35℃ than at the control temperature,whereas the activity was lower at 40℃ than the control.The strength of APX isozyme bands was also elevated at 30 and 35℃.A new band appeared at the 35℃.Both the strength and the number of the bands decreased at 40℃.The degenerate primers were designed according to amino acid sequences of cAPX from NCBI.A 752 bp specific fragment was obtained by the PCR amplification.Homologous comparation with other plants apx genes,the fragment obtained is highly conserved and contains the complete open reading frame.
Litchi chinensis high-temperature stress APX isozymes gene cloning
W.Shao Z.X.Lai Q.Huang Y.Q.Guo
Institute of Horticultural Biotechnology Fujian Agricultural and Forestry University Fuzhou,Fujian 350002 China
国际会议
福州
英文
183-187
2008-08-25（万方平台首次上网日期，不代表论文的发表时间）