POD Isoenzyme and RAPD Analysis on Litchi in Fuqing
The paper discussed the genetic relationship between superior individual late-maturity Yurongwanli and other cultivars. To do so POD isoenzyme and RAPD technology were used to analyze iitchi germplasm resources of Fuqing city. There were 4 bands produced by POD isoenzyme electrophoresis. According to the differences of these bands,Shishengshu1,Shishengshu2,Xiafanzhi and Dongliuyihao were put in the frist category,while Shangfanzhi and Yuanhong were placed in the second category. RAPD-PCR analysis showed that seven primers produce a total of 59 bands,with 8.43 fragments per prime.Among these 59 bands 40 were polymorphic,with a 67% polymorphism rate. The DPS2000 software was used to analyze the RAPD genotype date matrix. The result showed that the 7 cultivars can be divided into two categories when the genetic distance D value is 0.70 and four categories when the D value is 0.60.These two results indicated that Yurongwanli has the closest genetic relationship with Hongxiuqiu.
litchi POD isoenzyme RAPD analysis cluster analysis
L.S.Liu D.M.Pan F.L.Zhong J.B.Wang
Commercial Crop Station Agriculture bureau of Fuqing Fuqing,Fujian,3503002 China College of Horticulture Institute of Storage Science and Technology of Horticultural Products Fujian
国际会议
福州
英文
189-193
2008-08-25(万方平台首次上网日期,不代表论文的发表时间)