Automatic cell cutting and nucleus detection

摘要：

We have developed an automated cell cutting and its nucleus detection technique for automated nucleus transplantation. Animal cells softened by cytochalasin treatment were injected into a microfluidic chip. The microfluidic chip contained 2 orthogonal channels: 1 microchannel was wide, was used to transport cells, and to generate the cutting flow; the other microchannel was thin and used for aspiration, fixing, and stretching of the cell. The injected cell was aspirated and stretched thin into the thin microchannel. Simultaneously, the volume of the cell before and after aspiration was calculated; this volume was used to calculate the fluid flow required to aspirate half the volume of the cell into the thin microchannel. Then, we applied a high-speed flow in the orthogonal microchanel to bisect the cell. Finally, we observed the nucleus of the cut cells to detect the non-nucleus cell. This paper reports the cutting and the detection process and the system, and result of the experiment.

作者: Akihiko Ichikawa Tamio Tanikawa Satoshi Akagi Kohtaro Ohba

作者单位: RT-Synthesis Research Group,Intelligent Systems Research Institute,National Institute of Advanced In Reproductive Biology and Technology Research Team,National Institute of Livestock and Grassland Scie

会议类型: 国际会议

会议名称: 2009 IEEE International Conference on Robotics and Biomimetics(2009 IEEE 机器人与仿生技术国际会议 ROBIO 2009)

会议地点: 桂林

会议语种:英文

页码: 153-158

在线出版日期: 2009-12-19（万方平台首次上网日期，不代表论文的发表时间）