A Microfluidic System for Semicontinuous Bead-Based Biosensing Platforms

摘要：

Conventional bead-based micro immunoassays do not guarantee high reliability because of the working principle for the trapping methods which cause inconsistency in trapping of cytometric beads. This paper presents a new bead-based micro immunoassay system for multiple or semicontinuous assays with high reliability and accuracy using hole-patterned structures and double-cladding optical fibers. Trapping test showed selective trapping of beads and consistency of the number of the trapped beads over repeated tests. Immunoassay using IL-6 showed that the cytometric beads were trapped in ordered fashion and provided a detection limit on the order of pg/ml.

关键词： (170.0170) Medical optics and biotechnology (170.2520) Fluorescence microscopy

作者: Hye Won Kim Young Man Kim Sung Keun Yoo Sang Youl Yoon Jong Hyun Lee Sung Yang

作者单位: Department of Mechatronics, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro (Oryon Graduate Program of Medical System Engineering, Gwangju Institute of Science and Technology, 261 Che Department of Mechatronics, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro (Oryon

会议类型: 国际会议

会议名称: ACP2009亚太光纤通信与光电国际会议

会议地点: 上海

会议语种:英文

页码: 1-2

在线出版日期: 2009-11-01（万方平台首次上网日期，不代表论文的发表时间）