PROTEOMIC ANALYSIS OF POULTRY LIVER IN REGION EXPOSED TO DIOXIN AT MA DA DISTRICT, DONG NAI PROVINCE, VIETNAM
Agent Orange is the code name for a powerful herbicide and defoliant used by US military in its Herbicidal Warfare program during the Vietnam War from 1961 to 1971. Agent Orange contains TCDD (2,3,7,8- tetrachlorodibenzo-p-dioxin) that has a harmful influence on people and organisms in the exposed regions, the most serious effects concentrated in the areas, especially in Ma Da, Dong Nai. In this research, we carried out proteomic analysis of poultry liver in region exposed to dioxin (Ma Da, Dong Nai) and Hanoi as control. Two-dimensional electrophoresis (2-DE) was utilized to resolve the protein profile of poultry liver at different regions. Some notably different spots between Ma Da and control gels were selected for protein detection by MALDI-TOF MS PMF. Among identified proteins in Ma Da gels, proteins, which play important roles in growth and development processes, in tumor or cell proliferation suppression and stresses, are noticeable. These are Hox9_Z4, Zinc finger protein, EphB, Tyrosine kinase receptor B, Novel ITAM-containing IgSF receptor 2…Especially heat shock protein 70 kDa, a chaperon protecting organisms against heat stresses or toxic chemicals, was expressed; this protein only obviously expressed in Muscovy duck liver belong to Ma Da.
Ngo T.Mai Hoang T.T.Dung Trinh H.Thai
Structural Biology and Proteomics Lab.,Key Lab. for Enzyme-Protein Technology,College of Science,Vietnam National University,Hanoi,Vietnam
国际会议
北京
英文
1-4
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）