Preliminary Assessment of Bioaccessibility of HBCDs from Human GIT following Indoor Dust Ingestion Using a Physiologically Based Extraction Test (PBET)
An in vitro PBET which incorporates human gastrointestinal tract (GIT) parameters (including pH and chemistry, solid-to-solution ratio, mixing and emptying rates) was applied to study the bioaccessibility of α-, β- and γ-HBCDs from the 3 main GIT compartments following ingestion of indoor dust. While the oral compartment was not studied due to the short residence time in this compartment; 1 gram of a well-characterised, sieved and homogenised indoor dust sample was incubated at each of a simulated stomach (pH=2), small intestine (pH=7) and colon (pH=6.5) compartments for 1, 4 and 8 hours respectively. A control group was obtained from a similar experimental setup but no dust added. Samples were freeze dried, extracted using pressurised liquid extraction and analysed using LC-NIESI- MS/MS. No target compounds were above the detection limit (0.5 ng g-1) in the control group. Results revealed that the average bioaccessibility of γ-HBCDs from the 3 studied GIT compartments (51％) was less than that for α- and β-HBCDs (82％ and 69％ respectively) which is probably due to the lower aqueous solubility of the γ-isomer (2 μg L-1) compared to the α- and β-isomers (45 and 15 μg L-1 respectively). The average overall bioaccessibility of ∑HBCDs from the 3 GIT compartments studied was 77％. No significant change in the enantiomeric fractions was observed in any of the studied samples.
Mohamed Abou-Elwafa Abdallah Stuart Harrad Chris Collins Emma Tilston
Division of Environmental Health and Risk Management,Public Health Building,School of Geography,Eart Division of Environmental Health and Risk Management,Public Health Building,School of Geography,Eart Department of Soil Science,School of Human and Environmental Sciences,The University of Reading,Whit
国际会议
北京
英文
1-5
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）