A metabonomic method for profiling of metabolic changes in Hexabromocyclododecane and Tetrabromobisphenol A induced rat serum
This work characterizes the metabolism disorders of rat induced by TBBPA or HBCD in a Sprague Dawley rat model with two dosage of 30mg/kg and 300mg/kg. A seven-day long continuous administration was performed in male rats and sacrificed on Day 8 for serum collecting in this experiment. An integrated analytical approach based on Ultra performance liquid chromatography coupled with quatropde-time of flight mass spectrometer (UPLC-Q-TOF-MS) was developed to map the metabolic response in serum. LC-MS spectra of the serum were analyzed via pattern recognition using partial least squares discriminant analysis (PLS-DA). The PLS-DA score plot presented distinct separation between control group and dosed groups of TBBPA or HBCD. It was demonstrated that the metabolic patterns of the rats have been disturbed after oral dosed. The metabonomic approach based on LC–MS indicated several kinds of endogenous metabolites as biomarkers in serum associated with metabolic disorders induced by TBBPA or HBCD. The present study proves the great potential of LC–MS based metabonomics in mapping metabolic response for toxicology.
Feng J F Shi Z X Wu Y N Li J G Zhao Y F Shao B Duan H J
School of Public Health and Family Medicine,Capital Medical University,Beijing 100069,China School of Public Health and Family Medicine,Capital Medical University,Beijing 100069,China National National Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention,Be Beijing Center for Disease Control and Prevention,Beijing 100011,China
国际会议
北京
英文
1-6
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）