ROBUSTESS OF THE CALUX BIOASSAY: STATISTICAL AALYSIS OF THE BETWEE-WELL VARIABILITY FOR THE H1L6.1C3 MOUSE HEPATOMA CELL LINE

摘要：

The variation of the CALUX response between different wells on a 96-well plate was investigated for the H1L6.1c3 mouse hepatoma cell line. Analysis of 6 plates, spiked with 1.5 pg/μL of a 2, 3, 7, 8 -TCDD standard solution, on 3 different days indicated significant differences in RLU values between the wells. The mean RLU values were significantly lower (p = 1.43E-12) in the outer circle of the plate (i.e. rows A and H, and columns 1 and 12) and there were significant differences found between both columns and rows in a two-way ANOVA (p < 0.001). Even after rejection of the outer wells of the plate, significant differences were still found between the rows, columns (both p-values < 0.001) and circles (p = 0.002). Therefore, it is important to pay attention to the spatial distribution of the samples, standards and blanks on the plate and to take into account the between-well variability on a 96-well plate.

作者: Croes K Van Langenhove K Elskens M Goeyens L Baeyens W

作者单位: Vrije Universiteit Brussel (VUB),Department of Analytical and Environmental Chemistry,Pleinlaan 2,1050 Brussels,Belgium

会议类型: 国际会议

会议名称: 29th International Symposium on Halogenated Persistent Organic Pollutants(第29届国际持久性有机污染物研讨会)(2009国际二噁英大会)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-08-24（万方平台首次上网日期，不代表论文的发表时间）