TOF-SIMS MASS SPECTROMETRY IMAGING DEMONSTRATES A SELECTIVE TROPISM OF BDE-209 RESIDUES LOCATION IN TARGET TISSUES OF RATS
BDE-209 (Deca-Bromo-Diphenyl Ether, DBDE) is a major BFR for which human exposure has been demonstrated. DBDE, administered by oral route, is efficiently absorbed in rats (at least 20％ of the dosage) and its residues are mainly concentrated in the liver and in two endocrine glands, namely the adrenals and the ovaries. In this study, we based on previously published work and dosed female rats 2 mg/kg BW DBDE daily over 96 hr, with the aim to investigate the possible tropism of DBDE residues in these target tissues. We took advantage of the presence of bromine atoms to check the possibility to detect DBDE residues using a novel technique of imagery: Time-of-Flight Secondary Ion Mass Spectrometry. ToF-SIMS mass spectrometry imaging proved to be an efficient method for the study of the localisation of DBDE residues. It allowed to reveal an homogeneous repartition of DBDE residues in the livers of dosed rats. In ovaries, residues were located in several spots, possibly corresponding to corpora lutea. In addition, the study of the repartition of DBDE residues in the adrenals showed a marked cortical tropism. Due to the intrinsic toxicity of DBDE, but also because this BFR is metabolised into several BDE with a lower degree of bromination in vivo, part of which could be biologically active metabolites, it appears that DBDE residues repartition in the adrenals and the ovaries may be connected with the mechanisms of action by which BDE could trigger endocrine disruption in mammals.
Seyer A Riu A Debrauwer L Laprévote O Bourges N Brunelle A Zalko D
Institut de Chimie des Substances Naturelles,CNRS,UPR 2301,Avenue de la Terrasse,91190 Gif-sur-Yvett UMR 1089 Xénobiotiques INRA-ENVT,180 Chemin de Tournefeuille,BP93173,F31027 Toulouse Cedex 3,France Institut de Chimie des Substances Naturelles,CNRS,UPR 2301,Avenue de la Terrasse,91190 Gif-sur-Yvett Ecole Nationale Vétérinaire de Toulouse ENVT,23 chemin des Capelles,BP 87614,31076 Toulouse,France
国际会议
北京
英文
1-5
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）