AEROBIC BIOTRANSFORMATION OF FLUOROAROMATIC COMPOUNDS BY PSEUDONOCARDIA BENZENIVORANS
Widespread usage of fluoroaromatic compounds as agrochemicals and pharmaceuticals has lead to an increased appearance of fluorine-containing pollutants. Therefore it is essential to understand its fate and accumulation potential in the environment. Compared with chlorinated and brominated compounds, sparse information about metabolism of fluorinated compounds by microorganisms is available. In this study, we found that Pseudonocardia benzenivorans which was previously isolated from an enrichment based on tetrachlorobenzene has broad metabolic diversity for monofluoroaromatic compounds. P. benzenivorans mineralized 4-fluorophenol (4-FP) as a sole source of carbon and energy. Besides, this strain could also transform other mono-fluorophenols (mono-FPs) via monooxygenase activity. The metabolic route of FP isomers was investigated using enzyme assay and isolation of intermediates by high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). Two forms of fluorocatechol were revealed as the key degradation products during mono-FPs biotransformation. This is first comprehensive report about metabolic process of all mono-FPs by a single bacterium.
Kim E J Kim Y M Jeon J R Kumarasamy M Chang Y S
School of Environmental Science and Engineering,Pohang University of Science and Technology (POSTECH),Pohang,790-784,Korea
国际会议
北京
英文
1-4
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）