Simultaneous determination of dioxins and all PCB isomers in food samples using accelerated solvent extraction and gel permeation chromatography
We developed a simultaneous rapid analysis method for determination of 17 kinds of toxic PCDD/F isomers and 209 kinds of PCB isomers in food samples. This method makes it possible to evaluate total TEQ and total PCB concentration with a single extract operation. The method consists of four processes, as follows: (i) rapid and high-efficiency extraction using accelerated solvent extraction (ASE), (ii) sample cleanup using modified multi-layer silica gel column chromatography and active carbon column chromatography, (iii) high-efficiency cleanup by gel permeation chromatography (GPC), (iv) isomer specific determination using high-resolution gas chromatography coupled with high-resolution mass spectrometry (HRGC/HRMS). In this study, we examined the conditions needed for multi-layer silica gel column chromatography and GPC to remove fat and other matrices from extracts effectively. We evaluated the performance of the method using data from a standard recovery test and a quantitative reproduction test using a fish sample purchased in a market. The mean recovery rates for a labeled standard for 26 kinds of PCBs ranged from 46％ to 117％, which is within the range officially recommended for dioxins. RSD (％) values for PCB concentrations are below 20％.
Hori T Yasutake D Ashizuka Y Kajiwara J Nakagawa R Yoshimura T Tsutsumi T
Fukuoka Institute of Health and Environmental Sciences,39 Mukaizano,Dazaifu-shi,Fukuoka,818-0135,Jap National Institute of Health Sciences,1-18-1 Kamiyoga,Setagaya-ku,Tokyo 158-8501,Japan
国际会议
北京
英文
1-5
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）