AH RECEPTOR AGONISTS PRESENT IN COMMERCIAL AND CONSUMER PRODUCTS: IDENTIFICATION AND CHARACTERIZATION OF NOVEL AGONISTS PRESENT IN TIRE EXTRACTS
The Ah receptor (AhR) is known to mediate the toxic and biological effects of a wide variety of structurally diverse chemicals. Application of the CALUX bioassay for the detection of AhR agonists in environmental, biological and many other matrices has led to the identification of novel chemicals and classes of chemicals that can bind to and activate the AhR. Since the presence of AhR agonists in extracts of these matrices can interfere with clear interpretation of CALUX assay results, their identification and characterization is necessary. Here we have demonstrated the presence of AhR agonists in solvent extracts of truck tire tread using both CALUX and AhR DNA binding bioassays. The application of bioassay-based fractionation coupled with instrumental analysis not only led to the isolation and identification of several known polycylic aromatic hydrocarbons which have been previously shown to activate the AhR signaling pathway, but it also identified 2-mercapto- and 2-hydroxybenzothiazole as AhR agonists.
Guochun He Bin Zhao Michael S.Denison
Department of Environmental Toxicology,Meyer Hall,University of California,Davis,CA 95616,USA Department of Environmental Toxicology,Meyer Hall,University of California,Davis,CA 95616,USA Resear
国际会议
北京
英文
1-5
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）