Synergistic Cytotoxicity between Pentachlorophenol and Copper-1,10-phenanthroline Complex in Human Liver Cells
The combination effects of pentachlorophenol (PCP) and copper-1,10-phenanthroline(Cu(OP)2) on the human has been gradually concerned. Our previous study have shown that combination of PCP and Cu(OP)2 induce synergistic cytotoxicity in E.coli. However, this effect is still not investigated in human cell. The present study is performed to investigate whether the combination of PCP and Cu(OP)2 cause the same synergistic cytotoxic effects on both HL-7702 and HepG2 cells as observed in E.coli and further examine the possible mechanism. In the present study, (PCP)2Cu(II)(OP)2 cause cytotoxicity of both HL-7702 and HepG2 cells in a time- and concentration-dependent manner and HepG2 cells are more susceptible to (PCP)2Cu(II)(OP)2-induced cell death compared with normal HL-7702 cells. (PCP)2Cu(II)(OP)2-induced cell death of HepG2 cells was mainly due to apoptosis, which is associated with the decrease of mitochondrial membrane potential and anti-apoptotic proteins, release of cytochrome c and activation of caspase-3, 9. Furthermore, it has been demonstrated that (PCP)2Cu(II)(OP)2 significantly cause the increase of ROS level and decrease of the GSH/GSSG ratio. In conclusion, the combination of both non-toxic levels of PCP and Cu(II)(OP)2 results in synergistic cytotoxicity of both normal HL-7702 and HepG2 cells, which was characterized by ROS-mediated apoptosis in a mitochondrial dependent manner.
Sheng Z.G Zhu B.Z
State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Science,Chinese Academy of Sciences,Beijing 100085,Peoples Republic of China
国际会议
北京
英文
1-6
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）