LC-MS ANALYSIS OF TRICLOSAN AND ITS METABOLITES: DIOXINS RELATED?
Triclosan is a widely used broad-spectrum antibacterial agent by specifically inhibiting enoyl–acyl carrier protein reductase. The oral metabolism of triclosan was investigated on SD rat, while in vitro metabolic study was performed using SD rat liver S9 and microsome. Fourteen metabolites were identified by using in-source fragmentation from high performance liquid chromatography-negative atmospheric pressure chemical ionization/ion trap mass spectrometry (HPLC-APCI/ITMS). The results of in vivo metabolism indicated that the glucuronidation and sulfonation were the major pathway of phase II metabolism and the hydroxylated products were the major phase I metabolites. Moreover, glucose, mercapturic acid and cysteine conjugates of triclosan were also observed in the urine samples of rats orally administrated with triclosan. The in vitro metabolic rates of triclosan and its major glucuronidation and sulfonation metabolites were determined by isotope dilution high performance liquid chromatography-electrospray/ion trap mass spectrometry (HPLC-ESI/ITMS) method.
Jian-lin Wu Zongwei Cai
Department of Chemistry,Hong Kong Baptist University,Kowloon Tong,Kowloon,Hong Kong
国际会议
北京
英文
1-3
2009-08-24（万方平台首次上网日期，不代表论文的发表时间）