Influence of Pore Size on Enzymes Adsorption in Mesostructure Cellular Foams (MCFs)
Mesoporous silica materials are attractive candidates for enzymes immobilization due to their high surface area, tunable pore size, large pore volume and biocompatibility. In this work, two different enzymes, papain, a small globular enzyme with molecular diameter of 3.6nm, and catalase, a relatively larger enzyme with molecular diameter of 10.4nm were introduced into the pores of siliceous mesostructed cellular foams (MCFs) that had a large cellular pores of 29nm and a small window size of 12nm, respectively. The amount of adsorbed enzymes was found to be dependent on the molecular size of enzymes. The amount of adsorbed catalase was more than two times that of adsorbed papain, suggesting that MCFs with large pores is a suitable host for large enzymes adsorption. The blocking of pores which resulted from aggregation of enzymes in the windows of MCFs and the higher leaching of enzymes form MCFs may be the reason of lower papain adsorption capacity in MCFs.
Enzymes adsorption Mesostructured cellular foams Pore size Enzymes size
Wei NA Qi WEI ZeChang ZOU ZhiHong WANG QunYan LI ZuoRen NIE
College of Materials Science and Engineering, Beijing University of Technology, Beijing 100124
国际会议
重庆
英文
109-113
2008-06-09（万方平台首次上网日期，不代表论文的发表时间）