Stimuli-responsive Materials Applied as Carriers of Immobilized Enzymes
Enzymes, immobilized on pH/temperature/ion-responsive carriers, relieve poor contact and could be conveniently recycled as well. Enzymes were immobilized on pH-responsive carriers often by covalent method, which was better than non-covalent method (adsorption). The immobilized enzymes showed slight steric hindrance for enzymatic reaction, but always lose much activity during recycle process. The Km values were often increased significantly via immobilization on temperature/ion responsive carriers, while the immobilized enzymes expressed superior reusability. Both covalent and non-covalent methods were fit for enzymes to be immobilized on temperature-responsive carriers. Non-covalent method (entrapment) was preferential choose for enzymes to be immobilized on ion-responsive carriers. Many drawbacks, such as large activity loss and slow respondence during stimulus occurrence, seriously restrained the effect of the immobilized enzymes. It was supposed to open up special stimuli-responsive materials for enzymes immobilization, and choose proper immobilization methods, correspondingly.
Stimuli-responsive Materials pH-responsive Ion-responsive Temperature-responsive Immobilized Enzymes
Yu ZHANG Jingliang XU Zhenhong YUAN
Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences; Key Laboratory of Renewable E Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences; Key Laboratory of Renewable E
国际会议
重庆
英文
1198-1202
2008-06-09（万方平台首次上网日期，不代表论文的发表时间）