Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix
The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.
Mesenchymal Stem Cell Vascular Endothelial Growth Factor Gene Therapy Acellular Dermal Matrix Tissue Engineering
Xiangrong ZHANG Dewu LIU Guanghua GUO Yan PENG
Department of Ophthalmology, First Affiliated Hospital of Nanchang University,Nanchang,China Department of Burns, First Affiliated Hospital of Nanchang University, Nanchang, China
国际会议
重庆
英文
1298-1301
2008-06-09（万方平台首次上网日期，不代表论文的发表时间）