Construction of Two-gene Modified Artificial Bone by Using Recombinant Adenovirus
Large osseous defects are difficult to treat because of deficient blood supply on the defected area. To get sufficient blood supply, we designed to establish the adenovirus simultaneously encoding both VEGF and Ang-1 (pAd-VIA) to accelerate the formation of new vessels in the process of bone defect repair. The construction of the adenovirus was performed according to the method reported by Tong-Chuan HE with a tiny modification. Three kinds of adenoviruses were acquired. They are adenovirus pAd-VIA simultaneously encoding VEGF and Ang-1, adenovirus pAd-VEGF encoding VEGF, and adenovirus pAd-Ang-1 encoding Ang-1. The adenovirus prepared in this study could successfully transfer VEGF and Ang-1 into mesenchymal stem cells(MSCs) with high efficiency. Two-gene modified artificial bone was established by use of these adenovirus. In the end, the two-gene modified artificial bone was proved to have good biocompatibility and biological function. Study reports presented here will pave the way for further exploration of vascularization in the process of large osseous defects repair.
VEGF Ang-1 Gene Transfer Artificial Bone
Chunguang DUAN Guolin MENG Jian LIU Yunyu HU Baofeng LI Dan LI Jianping BAI Long BI Min LIU
Institute of Orthopaedics & Traumatology, Xijing Hospital,Fourth Military Medical University, Xian , China
国际会议
重庆
英文
1343-1355
2008-06-09（万方平台首次上网日期，不代表论文的发表时间）